ANA test kit

Catalogue Number: MC-57006

Application: To identify the presence of anaerobic bacteria

this method is used in various industries such as oil, gas, petrochemicals, aviation, food, water and wastewater, and other industries with specific formulations for testing different samples such as drinking water, sewage, and food samples.

click here for more information: Microbes as Corrosion Agents in Facilities | Microbial Corrosion in Metals | Rapid Identification Key to Combat Corrosion | Systems Prone to Microbial Contamination!

 

توضیحات

 

Anaerobic bacteria are a diverse group of heterotrophic bacteria that are divided into three categories based on their oxygen requirements. Firstly, obligate anaerobic bacteria that do not grow in the presence of oxygen. Secondly, facultative anaerobic bacteria that can grow in the presence or absence of oxygen. And thirdly, microaerophilic bacteria that require very small amounts of oxygen for growth, but have better growth in its absence. Anaerobic bacteria include both gram-positive and gram-negative types, and can grow in a wide range of environments. Two major concerns of bacterial growth are clinical microbial infections and microbial decay.

Test

Test

Initial recommendations

  • Avoid contacting the inner wall of the Falcon tube and its lid. Perform the test under sterile conditions.
  • After opening the Falcon tube, invert it so that its bottom faces the ground and place it on a clean surface.
  • Do not shake or rotate the Falcon tube after the sample has been added. Allow the ball to be placed on the floating surface.
  • Many anaerobic bacteria do not grow directly in water, but rather grow in depths and biofilms. Ensure that you collect samples from suitable sites.

 

Test

  • Collect at least 20 milliliters of sample.
  • Pour 19 milliliters of the sample into the test tube and close the lid tightly.
  • Write down the date and name of the sample on the kit label and attach it to the falcon tube.
  • Incubate the test tube at 37 degrees Celsius in an incubator or at room temperature (21-25 degrees Celsius) and away from sunlight.
  • Observe the sample every day for 8 days. Note the date of the first observed reaction.

 

results interpretation

results interpretation

Detection of the presence/absence of bacteria

The medium inside the Falcon tubes of this kit contains a rich culture medium to support the growth of various anaerobic bacteria. Due to the placement of the ball on the surface of the medium, the conditions inside the Falcon tube become anaerobic. The lower part of the Falcon tube is completely anaerobic, and gradually, from the bottom to the top of the ball, the degree of anaerobicity decreases. Obligate anaerobic bacteria grow in the lower part of the Falcon tube, and the bacterial population that will grow around the ball is mostly facultative anaerobes. Obligate anaerobic bacteria grow in the lower part of the Falcon tube, and the bacterial population that will grow around the ball is mostly facultative anaerobes. Microaerophillic bacteria will also grow along the length of the Falcon tube.

If any anaerobic bacteria are present in the sample added to the kit, bacterial growth will be visible in the medium by creating turbidity 1 to 8 days after inoculation.

 

Estimating the approximate population and aggressive potential of bacteria:

If the test result is positive, you can estimate the bacterial population and the extent of their invasion according to the tables below. A faster response occurs when the population of bacteria is higher.

 

ANAtable02-ibresco

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

توضیحات

 

Anaerobic bacteria are a diverse group of heterotrophic bacteria that are divided into three categories based on their oxygen requirements. Firstly, obligate anaerobic bacteria that do not grow in the presence of oxygen. Secondly, facultative anaerobic bacteria that can grow in the presence or absence of oxygen. And thirdly, microaerophilic bacteria that require very small amounts of oxygen for growth, but have better growth in its absence. Anaerobic bacteria include both gram-positive and gram-negative types, and can grow in a wide range of environments. Two major concerns of bacterial growth are clinical microbial infections and microbial decay.

Test

Test

Initial recommendations

  • Avoid contacting the inner wall of the Falcon tube and its lid. Perform the test under sterile conditions.
  • After opening the Falcon tube, invert it so that its bottom faces the ground and place it on a clean surface.
  • Do not shake or rotate the Falcon tube after the sample has been added. Allow the ball to be placed on the floating surface.
  • Many anaerobic bacteria do not grow directly in water, but rather grow in depths and biofilms. Ensure that you collect samples from suitable sites.

 

Test

  • Collect at least 20 milliliters of sample.
  • Pour 19 milliliters of the sample into the test tube and close the lid tightly.
  • Write down the date and name of the sample on the kit label and attach it to the falcon tube.
  • Incubate the test tube at 37 degrees Celsius in an incubator or at room temperature (21-25 degrees Celsius) and away from sunlight.
  • Observe the sample every day for 8 days. Note the date of the first observed reaction.

 

results interpretation

results interpretation

Detection of the presence/absence of bacteria

The medium inside the Falcon tubes of this kit contains a rich culture medium to support the growth of various anaerobic bacteria. Due to the placement of the ball on the surface of the medium, the conditions inside the Falcon tube become anaerobic. The lower part of the Falcon tube is completely anaerobic, and gradually, from the bottom to the top of the ball, the degree of anaerobicity decreases. Obligate anaerobic bacteria grow in the lower part of the Falcon tube, and the bacterial population that will grow around the ball is mostly facultative anaerobes. Obligate anaerobic bacteria grow in the lower part of the Falcon tube, and the bacterial population that will grow around the ball is mostly facultative anaerobes. Microaerophillic bacteria will also grow along the length of the Falcon tube.

If any anaerobic bacteria are present in the sample added to the kit, bacterial growth will be visible in the medium by creating turbidity 1 to 8 days after inoculation.

 

Estimating the approximate population and aggressive potential of bacteria:

If the test result is positive, you can estimate the bacterial population and the extent of their invasion according to the tables below. A faster response occurs when the population of bacteria is higher.

 

ANAtable02-ibresco

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen