Bile Esculin Agar

Application: A differential medium for differentiating enterococci and streptococcus bovis group from other streptococci.

Catalog Number: i23065

توضیحات

Preparation

Shake the container well. Dissolve 43.5 grams of the powder in one liter of distilled water and mix thoroughly.
Heat it gently and boil for one minute to completely dissolve the medium.
Autoclave the culture medium at 121 degrees Celsius for 15 minutes.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Inoculate the samples in a culture plate and incubate for 24-48 hours under aerobic conditions at a temperature of 35±2 degrees Celsius.

Results and Interpretation

If the cultivation is done on a plate, any blackening of the color of the cultivation medium in the plate indicates a positive reaction, and the lack of blackening indicates a negative reaction.
In cases where the sample has been inoculated in the form of a slant in the prepared cultivation medium, if more than half of the slant has turned black after 24-48 hours, the test result will be positive, and if less than half or none of it has turned black after this period, the test result will be negative.

Note

Strains of lactococcus, leuconostoc, and pediococcus bacteria isolated from human infections have had a positive reaction in this cultivation medium.
Rare strains of viridans streptococci are capable of blackening the bile esculin agar cultivation medium and creating a weak positive reaction.

پرسش و پاسخ

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Quality Control

Dehydrated Appearance: Fine, homogeneous, free of extraneous material, may contain a moderate amount of very small dark particles.
Prepared Appearance: Dark, tan olive to olive green with a blue tint, trace hazy to hazy.
Cultural response was observed after 42-48 hours of incubation at 35 ± 2°C.

Reaction	Growtht	ATCCt	Standard Strain
Black Colonies	Good	29212	Enterococcus faecalis
-	Partial to Complete Inhibitiont	19615	Sterptococcus pyogenes

Components

gr/Lit	Components
5.0	Pancreatic Digest of Gelatin
3.0	Beef extract
20.0t	Oxgall
0.5t	Ferric citrate
1.0t	Esculin
14.0t	Agar
0.2 ± 6.8t	Final pH

توضیحات

Preparation

Shake the container well. Dissolve 43.5 grams of the powder in one liter of distilled water and mix thoroughly.
Heat it gently and boil for one minute to completely dissolve the medium.
Autoclave the culture medium at 121 degrees Celsius for 15 minutes.

STORAGE:

Test

Inoculate the samples in a culture plate and incubate for 24-48 hours under aerobic conditions at a temperature of 35±2 degrees Celsius.

Results and Interpretation

If the cultivation is done on a plate, any blackening of the color of the cultivation medium in the plate indicates a positive reaction, and the lack of blackening indicates a negative reaction.
In cases where the sample has been inoculated in the form of a slant in the prepared cultivation medium, if more than half of the slant has turned black after 24-48 hours, the test result will be positive, and if less than half or none of it has turned black after this period, the test result will be negative.

Note

Strains of lactococcus, leuconostoc, and pediococcus bacteria isolated from human infections have had a positive reaction in this cultivation medium.
Rare strains of viridans streptococci are capable of blackening the bile esculin agar cultivation medium and creating a weak positive reaction.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Dehydrated Appearance: Fine, homogeneous, free of extraneous material, may contain a moderate amount of very small dark particles.
Prepared Appearance: Dark, tan olive to olive green with a blue tint, trace hazy to hazy.
Cultural response was observed after 42-48 hours of incubation at 35 ± 2°C.

Reaction	Growtht	ATCCt	Standard Strain
Black Colonies	Good	29212	Enterococcus faecalis
-	Partial to Complete Inhibitiont	19615	Sterptococcus pyogenes

Components

gr/Lit	Components
5.0	Pancreatic Digest of Gelatin
3.0	Beef extract
20.0t	Oxgall
0.5t	Ferric citrate
1.0t	Esculin
14.0t	Agar
0.2 ± 6.8t	Final pH

Bile Esculin Agar

Preparation

STORAGE:

Test

Test

Results and Interpretation

Note

Quality Control

Preparation

STORAGE:

Test

Test

Results and Interpretation

Note

Quality Control

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CoA & MSDS files

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CoA & MSDS files

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