Bile Esculin Azide Agar

Application: for isolation and enumeration of enterococcal bacteria (fecal streptococci)

Standards: ISO

Catalog Number:i23017

توضیحات

Preparation

Shake the con tainer of Bile Esculin Azide Agar well. Dissolve 54.65 grams of the powder medium in one liter of distilled water and mix thoroughly.
Heat it gently and boil for one minute to completely dissolve the medium.
Autoclave the culture medium at 121 degrees Celsius for 15 minutes.
The prepared medium can be stored in a plate at a temperature of 2-8°C for up to 2 weeks.

STORAGE:

Store the powder in the bottle tightly closed and below 30°C and the prepared medium in the tubes at 2-8°C. The best time to use is before the expiration date printed on the product label.

Test

In accordance with the type of sample and the relevant standard protocol, inoculate the plate containing the culture medium.
Incubate the culture plates at a temperature of 35 ± 2°C for 18-24 hours.

Results and Interpretation

If the cultivation is done on a plate, any blackening of the color of the cultivation medium in the plate indicates a positive reaction, and the lack of blackening indicates a negative reaction.
If the sample is cultured in the form of a slant, and if half of the slant has turned black after 24-48 hours, the result of the test is positive. If less than half of it has turned black after this period, or if it has not turned black at all, the test result will be negative.

پرسش و پاسخ

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Quality Control

Dehydrated Appearance: Cream to yellow, free-flowing, homogeneous, may contain some tan specks.
Prepared Appearance: Amber, clear to slightly opalescent.
Cultural response was observed after 24-48 hours of incubation at 35 ± 2°C.

Esculin Hydrolysis Reaction	Growth	ATCC	Standard Strain
black	Goodt	29212	Enterococcus faecalis
black	Good	19433t	Enterococcus faecalis
Colorless Colonies	Partial to Complete Inhibitiont	19615	Sterptococcus pyogenes
-	Inhibitiont	25922t	Escherichia coli

Components

gr/Lit	Components
17.0t	Peptone from Casein
3.0t	Peptones
5.0	Yeast Extract
5.5	Sodium Chloride
1.0	Esculin
0.5	Ammonium Iron (III) Citrate
10.0t	Ox Bile
0.15t	Sodium Azide
13.0	Agar
0.2 ± 7.1t	Final pH

توضیحات

Preparation

Shake the con tainer of Bile Esculin Azide Agar well. Dissolve 54.65 grams of the powder medium in one liter of distilled water and mix thoroughly.
Heat it gently and boil for one minute to completely dissolve the medium.
Autoclave the culture medium at 121 degrees Celsius for 15 minutes.
The prepared medium can be stored in a plate at a temperature of 2-8°C for up to 2 weeks.

STORAGE:

Store the powder in the bottle tightly closed and below 30°C and the prepared medium in the tubes at 2-8°C. The best time to use is before the expiration date printed on the product label.

Test

In accordance with the type of sample and the relevant standard protocol, inoculate the plate containing the culture medium.
Incubate the culture plates at a temperature of 35 ± 2°C for 18-24 hours.

Results and Interpretation

If the cultivation is done on a plate, any blackening of the color of the cultivation medium in the plate indicates a positive reaction, and the lack of blackening indicates a negative reaction.
If the sample is cultured in the form of a slant, and if half of the slant has turned black after 24-48 hours, the result of the test is positive. If less than half of it has turned black after this period, or if it has not turned black at all, the test result will be negative.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Dehydrated Appearance: Cream to yellow, free-flowing, homogeneous, may contain some tan specks.
Prepared Appearance: Amber, clear to slightly opalescent.
Cultural response was observed after 24-48 hours of incubation at 35 ± 2°C.

Esculin Hydrolysis Reaction	Growth	ATCC	Standard Strain
black	Goodt	29212	Enterococcus faecalis
black	Good	19433t	Enterococcus faecalis
Colorless Colonies	Partial to Complete Inhibitiont	19615	Sterptococcus pyogenes
-	Inhibitiont	25922t	Escherichia coli

Components

gr/Lit	Components
17.0t	Peptone from Casein
3.0t	Peptones
5.0	Yeast Extract
5.5	Sodium Chloride
1.0	Esculin
0.5	Ammonium Iron (III) Citrate
10.0t	Ox Bile
0.15t	Sodium Azide
13.0	Agar
0.2 ± 7.1t	Final pH

Bile Esculin Azide Agar

STORAGE:

Test

Test

Results and Interpretation

Quality Control

STORAGE:

Test

Test

Results and Interpretation

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

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