Blood Agar Base

Application: The prepared Blood Agar and Chocolate Agar culture media are useful for the identification and cultivation of many fastidious bacteria, especially pathogenic species, and for investigating their hemolytic reactions.

Standards: APHA

Catalog Number:i23020

توضیحات

Preparation

Shake the container well. Dissolve 40 grams of the culture powder in one liter of distilled water. Heat it gently until the media completely dissolves.
Autoclave at 121 degrees Celsius for 15 minutes.
After autoclaving, cool the media to 45-50 degrees Celsius, add 4-5% defibrinated sheep blood, and mix it well.
Before adding the blood, the prepared media should be clear and yellow-brown in color.
Prepared Blood Agar plates can be stored in the refrigerator for up to three months.

Prepare the Chocolate Agar culture media by boiling it.

After adding hemoglobin to the Blood Agar base media, boil and mix the culture media for 10 minutes at 80 degrees Celsius until it turns brown (chocolate color).

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Prepare the Blood Agar culture media according to the instructions. Add 5% defibrinated sheep blood to the media.
Place the desired sample on the culture plate and incubate at 35-37 degrees Celsius for 18-24 hours.
Note that to grow Streptococcus bacteria, culture media should be incubated in an incubator containing 3-5% carbon dioxide.

Results and Interpretation

The colony morphology of bacteria on Blood Agar containing 5% sheep blood is as follows:

Colonies of hemolytic streptococcal bacteria can be transparent or opaque and gray in color, seen as small (1 mm diameter) or large and mucoid (2-4 mm diameter) with a halo of hemolysis forming around them. Gram staining should be performed and macroscopic characteristics should be examined. (Some other organisms such as Listeria, some Corynebacteria, hemolytic Staphylococci, E. coli, and Pseudomonas also have hemolytic reactions.)
Pneumococcal bacteria colonies are usually broad, soft, transparent and gray in color, with a narrow greenish hemolysis halo (alpha hemolysis) sometimes seen around their mucoid colonies.
Staphylococci have opaque colonies that can be yellow-golden in color, with or without a beta-hemolysis halo.
Listeria bacteria are identified by their rod-shaped appearance in staining and motility at room temperature. A narrow beta-hemolysis halo is seen around their colonies.

Note

Colonies of Haemophilus haemolyticus bacteria on agar with horse or rabbit blood have beta-hemolysis and require a different method to differentiate them from streptococci. Using sheep blood is recommended because it lacks sufficient amounts of pyridine nucleotides, so Haemophilus haemolyticus bacteria do not grow on such media.

پرسش و پاسخ

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Quality Control

Dehydrated Appearance: Free flowing, homogeneous, beige.
Prepared Appearance: Plain – Medium, yellow to tan, clear to slightly hazy.
With 5% sheep blood – Cherry red, opaque.
Cultural Response: Prepare the medium per label directions without (plain) and with 5% defibrinated sheep blood (SB). Inoculate and incubate at 35 ± 2°C for 18-24 hours (incubate streptococci with 3-5% CO2).

Bacitracin Test	Growth in blood mediat	Growth in base mediat	ATCC	Standard Strain
	Good، No hemolysist	N/At	10231t	Candida albicans
	Good، Betat	N/At	19115t	Listeria monocytogenes
	N/At	Good	10145	Pseudomonas aeroginosa
	Good، Beta	Goodt	25923	Staphylococcus aureus
-	Good، Alphat	Good	6301	Streptococcus pneumoniae
+	Good، Betat	Goodt	12344	Streptococcus pyogenes

Components

gr/Lit	Components
20.0	Peptones
5.0	Sodium Chloride
15.0	Agar
0.2 ± 6.8t	Final pH

توضیحات

Preparation

Shake the container well. Dissolve 40 grams of the culture powder in one liter of distilled water. Heat it gently until the media completely dissolves.
Autoclave at 121 degrees Celsius for 15 minutes.
After autoclaving, cool the media to 45-50 degrees Celsius, add 4-5% defibrinated sheep blood, and mix it well.
Before adding the blood, the prepared media should be clear and yellow-brown in color.
Prepared Blood Agar plates can be stored in the refrigerator for up to three months.

Prepare the Chocolate Agar culture media by boiling it.

After adding hemoglobin to the Blood Agar base media, boil and mix the culture media for 10 minutes at 80 degrees Celsius until it turns brown (chocolate color).

STORAGE:

Test

Prepare the Blood Agar culture media according to the instructions. Add 5% defibrinated sheep blood to the media.
Place the desired sample on the culture plate and incubate at 35-37 degrees Celsius for 18-24 hours.
Note that to grow Streptococcus bacteria, culture media should be incubated in an incubator containing 3-5% carbon dioxide.

Results and Interpretation

The colony morphology of bacteria on Blood Agar containing 5% sheep blood is as follows:

Colonies of hemolytic streptococcal bacteria can be transparent or opaque and gray in color, seen as small (1 mm diameter) or large and mucoid (2-4 mm diameter) with a halo of hemolysis forming around them. Gram staining should be performed and macroscopic characteristics should be examined. (Some other organisms such as Listeria, some Corynebacteria, hemolytic Staphylococci, E. coli, and Pseudomonas also have hemolytic reactions.)
Pneumococcal bacteria colonies are usually broad, soft, transparent and gray in color, with a narrow greenish hemolysis halo (alpha hemolysis) sometimes seen around their mucoid colonies.
Staphylococci have opaque colonies that can be yellow-golden in color, with or without a beta-hemolysis halo.
Listeria bacteria are identified by their rod-shaped appearance in staining and motility at room temperature. A narrow beta-hemolysis halo is seen around their colonies.

Note

Colonies of Haemophilus haemolyticus bacteria on agar with horse or rabbit blood have beta-hemolysis and require a different method to differentiate them from streptococci. Using sheep blood is recommended because it lacks sufficient amounts of pyridine nucleotides, so Haemophilus haemolyticus bacteria do not grow on such media.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Dehydrated Appearance: Free flowing, homogeneous, beige.
Prepared Appearance: Plain – Medium, yellow to tan, clear to slightly hazy.
With 5% sheep blood – Cherry red, opaque.
Cultural Response: Prepare the medium per label directions without (plain) and with 5% defibrinated sheep blood (SB). Inoculate and incubate at 35 ± 2°C for 18-24 hours (incubate streptococci with 3-5% CO2).

Bacitracin Test	Growth in blood mediat	Growth in base mediat	ATCC	Standard Strain
	Good، No hemolysist	N/At	10231t	Candida albicans
	Good، Betat	N/At	19115t	Listeria monocytogenes
	N/At	Good	10145	Pseudomonas aeroginosa
	Good، Beta	Goodt	25923	Staphylococcus aureus
-	Good، Alphat	Good	6301	Streptococcus pneumoniae
+	Good، Betat	Goodt	12344	Streptococcus pyogenes

Components

gr/Lit	Components
20.0	Peptones
5.0	Sodium Chloride
15.0	Agar
0.2 ± 6.8t	Final pH

Blood Agar Base

Prepare the Chocolate Agar culture media by boiling it.

STORAGE:

Test

Test

Results and Interpretation

Note

Quality Control

Prepare the Chocolate Agar culture media by boiling it.

STORAGE:

Test

Test

Results and Interpretation

Note

Quality Control

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