توضیحات
Preparation
- Shake the container of bolton broth medium well.
- Dissolve 13.8 g in 500 ml of distilled water.
- Autoclave at 121°C for 15 minutes.
- Cool to 45 – 50°C. Aseptically add 25 ml lysed horse blood and the content of 1 vial of Bolton Broth Selective Supplement. Mix well and distribute the broth into sterile screw top containers.
- After the addition of the sample the space between screw top and broth should be approx. 2cm.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
