BPLS Agar

Brilliant-green Phenol-red Lactose Sucrose Agar

Application: The selective medium for isolating Salmonella, except for S. typhosa and Shigella, from pathological samples, feces, urine, food, etc.

Catalogue Number: i23470

توضیحات

Preparation

Weigh 57 grams of the powder and dissolve it in 1 liter of distilled water.
Heat it gently for 1 minute until the medium is completely dissolved.
Autoclave in 121 degree Celsius for 15 minutes.
After autoclave allow it to cool down to a temperature of 50 degrees Celsius and pour in strile petri dishes.

STORAGE:

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

Test

Inoculate the plate either directly with the sample or with samples taken from a rich culture medium.
Tests should be performed with culture media that have fewer inhibitors.
Incubate the inoculated plates at a temperature of 35 ± 2 degrees Celsius for 24 hours.

Results and Interpretation

Lactose and sucrose fermenting bacteria such as E. coli, Citrobacter, Klebsiella, cause the color of the medium to turn yellow and also form yellow colonies.
Bacteria that do not ferment lactose and sucrose such as Salmonella can be identified by the pink colony color on the red medium.
The growth of Staphylococcus aureus, Enterococcus faecalis, and Bacillus subtilis can be identified by the yellow color change of the medium and the formation of yellow colonies.

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Components

gr/Lit	Components
5	Peptone from meat
5	Peptone from casein
5	Meat extract
3	Sodium Chloride
2	di-sodium Hydrogen Phosphate
10	Lactose
10	Sucrose
0.08	Phenol red
0.0125	Brilliant green
12	Agar

Quality Control

Dehydrated Appearance: Pink, free-flowing, homogeneous

Prepared Appearance: clear and red.

Cultural response was observed after 24 hours of incubation at 35 ± 2˚C.

Culture medium	Colony color	Recovery Rate	ATCC	Organism
Yellow	Yellow	Good	25922	Escherichia coli
Red	Pink	Good	14028	Salmonella typhimurium
Yellow	Yellow	Not limited	25923	Staphylococcus aureus
Yellow	Yellow	Not limited	33186	Enterococcus faecalis
Yellow	Orange/Yellow	Not limited	6633	Bacillus subtilis

توضیحات

Preparation

Weigh 57 grams of the powder and dissolve it in 1 liter of distilled water.
Heat it gently for 1 minute until the medium is completely dissolved.
Autoclave in 121 degree Celsius for 15 minutes.
After autoclave allow it to cool down to a temperature of 50 degrees Celsius and pour in strile petri dishes.

STORAGE:

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

Test

Inoculate the plate either directly with the sample or with samples taken from a rich culture medium.
Tests should be performed with culture media that have fewer inhibitors.
Incubate the inoculated plates at a temperature of 35 ± 2 degrees Celsius for 24 hours.

Results and Interpretation

Lactose and sucrose fermenting bacteria such as E. coli, Citrobacter, Klebsiella, cause the color of the medium to turn yellow and also form yellow colonies.
Bacteria that do not ferment lactose and sucrose such as Salmonella can be identified by the pink colony color on the red medium.
The growth of Staphylococcus aureus, Enterococcus faecalis, and Bacillus subtilis can be identified by the yellow color change of the medium and the formation of yellow colonies.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/Lit	Components
5	Peptone from meat
5	Peptone from casein
5	Meat extract
3	Sodium Chloride
2	di-sodium Hydrogen Phosphate
10	Lactose
10	Sucrose
0.08	Phenol red
0.0125	Brilliant green
12	Agar

Quality Control

Dehydrated Appearance: Pink, free-flowing, homogeneous

Prepared Appearance: clear and red.

Cultural response was observed after 24 hours of incubation at 35 ± 2˚C.

Culture medium	Colony color	Recovery Rate	ATCC	Organism
Yellow	Yellow	Good	25922	Escherichia coli
Red	Pink	Good	14028	Salmonella typhimurium
Yellow	Yellow	Not limited	25923	Staphylococcus aureus
Yellow	Yellow	Not limited	33186	Enterococcus faecalis
Yellow	Orange/Yellow	Not limited	6633	Bacillus subtilis

BPLS Agar

Preparation

STORAGE:

Test

Test

Results and Interpretation

Quality Control

Preparation

STORAGE:

Test

Test

Results and Interpretation

Quality Control

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CoA & MSDS files

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CoA & MSDS files

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