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Brain Heart Infusion (BHI) Agar
Brain Heart Infusion (BHI) Agar
Brain Heart Infusion (BHI) Agar
Brain Heart Infusion (BHI) Agar
BHI-agar-ibresco-1030x1030-1.png

Brain Heart Infusion (BHI) Agar

Application: a general-purpose medium for cultivating a wide range of organisms such as bacteria, yeast, and fungi.

Standards: SMWW, EPA, AOAC, BAM,APHA, ISO

Catalog Number:i23022

توضیحات

Preparation

  • Shake the container well. Dissolve 52 grams of the medium powder in one liter of distilled water and mix thoroughly.
  • Boil the medium for 2 minutes until fully dissolved.
  • Autoclave the culture medium at 121 degrees Celsius for 15 minutes.
  • Before use, mix the medium well to evenly distribute any remaining precipitate.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Test

Test

Adding blood to the base culture medium provides many essential growth factors for fastidious fungi. Since many organisms require initial carbon dioxide for growth after inoculation onto the culture plate, it is better to incubate the inoculated plates in an environment with 10-5% carbon dioxide for 24-48 hours at a temperature of 35±2 degrees Celsius.

To isolate fungi from infected samples, it is better to use another non-selective culture medium alongside this medium and incubate at a temperature of 25-30 degrees Celsius. In addition, to isolate systemic mycotic fungi, two sets of culture media should be incubated, one at a temperature of 25-30 degrees Celsius and the other at a temperature of 35±2 degrees Celsius.

Results and Interpretation

After the incubation and growth of the fungi, the colonies should be examined for color and morphology and biochemical and serological tests should be used for better identification. All cultures should be examined weekly for fungal growth, and growth-negative plates can only be reported negative after 4-6 weeks of incubation.

The BHI Agar medium isolated in a tube is used for cultivating and maintaining pure microbial cultures.

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Quality Control

Quality Control

  • Dehydrated Appearance: Beige, free-flowing, homogeneous.
  • Prepared Appearance: Light to medium amber, slightly opalescent to opalescent with a flocculent precipitate.
  • Cultural Response: Medium was prepared per label directions without (plain) or with 5% defibrinated sheep blood (SB). Inoculated and incubated at 35 ± 2°C without or with 5-10% CO2 for 18-48 hours (A. brasiliensis aerobically at 30 ± 2°C for 18-72 hours) with organisms below.
Growth with Sheep Blood Growtht ATCC Standard Strain
Good
-
19115t
Listeria monocytogenes
Good
Good
25922t
Escherichia coli
Good
Good
25923
Staphylococcus aureus
Good
Good
6305t
Streptococcus pneumoniae
Good
Good
16404
Aspergillus brasiliensis (niger)
Components
gr/Lit Components
27.5
Nutrient substrate (brain extract, heart extract and peptones)
2.0t
D(+) glucose
5.0t
Sodium Chloride
2.5t
di-Sodium Hydrogen Phosphate
2.5
di-Sodium Hydrogen Phosphate
15.0
Agar
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توضیحات

Preparation

  • Shake the container well. Dissolve 52 grams of the medium powder in one liter of distilled water and mix thoroughly.
  • Boil the medium for 2 minutes until fully dissolved.
  • Autoclave the culture medium at 121 degrees Celsius for 15 minutes.
  • Before use, mix the medium well to evenly distribute any remaining precipitate.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Test

Test

Adding blood to the base culture medium provides many essential growth factors for fastidious fungi. Since many organisms require initial carbon dioxide for growth after inoculation onto the culture plate, it is better to incubate the inoculated plates in an environment with 10-5% carbon dioxide for 24-48 hours at a temperature of 35±2 degrees Celsius.

To isolate fungi from infected samples, it is better to use another non-selective culture medium alongside this medium and incubate at a temperature of 25-30 degrees Celsius. In addition, to isolate systemic mycotic fungi, two sets of culture media should be incubated, one at a temperature of 25-30 degrees Celsius and the other at a temperature of 35±2 degrees Celsius.

Results and Interpretation

After the incubation and growth of the fungi, the colonies should be examined for color and morphology and biochemical and serological tests should be used for better identification. All cultures should be examined weekly for fungal growth, and growth-negative plates can only be reported negative after 4-6 weeks of incubation.

The BHI Agar medium isolated in a tube is used for cultivating and maintaining pure microbial cultures.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Quality Control

  • Dehydrated Appearance: Beige, free-flowing, homogeneous.
  • Prepared Appearance: Light to medium amber, slightly opalescent to opalescent with a flocculent precipitate.
  • Cultural Response: Medium was prepared per label directions without (plain) or with 5% defibrinated sheep blood (SB). Inoculated and incubated at 35 ± 2°C without or with 5-10% CO2 for 18-48 hours (A. brasiliensis aerobically at 30 ± 2°C for 18-72 hours) with organisms below.
Growth with Sheep Blood Growtht ATCC Standard Strain
Good
-
19115t
Listeria monocytogenes
Good
Good
25922t
Escherichia coli
Good
Good
25923
Staphylococcus aureus
Good
Good
6305t
Streptococcus pneumoniae
Good
Good
16404
Aspergillus brasiliensis (niger)
Components
gr/Lit Components
27.5
Nutrient substrate (brain extract, heart extract and peptones)
2.0t
D(+) glucose
5.0t
Sodium Chloride
2.5t
di-Sodium Hydrogen Phosphate
2.5
di-Sodium Hydrogen Phosphate
15.0
Agar

CoA & MSDS files

Downloading CoA & MSDS files

 
 

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:To download the MSDS file

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BactiSave - Bacterial Preservation System
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CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

BactiSave - Bacterial Preservation System
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