Brilliant Green Agar (BGA)

Application: A highly selective medium for isolating Salmonella bacteria species, excluding Salmonella typhi from meat, food, and other samples.

Standards:ISO

Catalogue Number: i23311

توضیحات

Preparation

Shake the container well. Dissolve 50 grams of the medium powder in 1 liter of distilled water and mix thoroughly.
Boil the medium for 1 minute until completely dissolved.
Autoclave the culture medium at 121 degrees Celsius for 15 minutes.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Following the standard principles, inoculate the sample on the culture plate and obtain pure colonies.
It is recommended to use semi-selective and non-selective media alongside the mentioned culture medium to increase the chances of obtaining gram-negative bacteria, which are present in small amounts in the sample, and also to identify different populations of organisms present in the sample.
Incubate the plates away from light at a temperature of 35±2 for 24-18 hours. If the results are negative after 24 hours, continue the incubation for another 24 hours.

Results and Interpretation

After incubation, the expected results from the growth of organisms are as follows:

Salmonella species, excluding Salmonella typhi and Salmonella paratyphi: white to pink colonies surrounded by a red halo.
Salmonella typhi and Salmonella paratyphi: weak growth or no growth.
Shigella: weak growth or no growth.
E. coli, Enterobacter, and Klebsiella: yellow or greenish-yellow colonies surrounded by a greenish-yellow halo.
Proteus: weak growth or no growth.
Pseudomonas: Pink to reddish colonies.
Gram-positive bacteria: weak growth or no growth.

پرسش و پاسخ

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Quality Control

Dehydrated Appearance:

Dehydrated Appearance: Pink, free-flowing, homogeneous.
Prepared Appearance: Orange-brown, very slightly to slightly opalescent.
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours

Colony color	Growtht	ATCC	Standard Strain
Yellow-green	Weak to good	25922	Escherichia coli
Red	Good	13076	Salmonella enterica subsp. Enterica serotype Enteritidis
Red	None to poor	19430	Salmonella enterica subsp. enterica None to serotype Typhi
Red	Good	14028	Salmonella enterica subsp. Enterica serotype Typhimurium
–	Inhibition	25923	Staphylococcus aureus

Component

gr/Lit	Components
10.0t	Proteose Peptone
3.0	Yeast Extract
10.0t	Lactose
10.0t	Sucrose
5.0	Sodium Chloride
0.0125t	Brilliant Green
0.08t	Phenol Red
12.0	Agar
0.2 ± 6.9t	Final pH

توضیحات

Preparation

Shake the container well. Dissolve 50 grams of the medium powder in 1 liter of distilled water and mix thoroughly.
Boil the medium for 1 minute until completely dissolved.
Autoclave the culture medium at 121 degrees Celsius for 15 minutes.

STORAGE:

Test

Following the standard principles, inoculate the sample on the culture plate and obtain pure colonies.
It is recommended to use semi-selective and non-selective media alongside the mentioned culture medium to increase the chances of obtaining gram-negative bacteria, which are present in small amounts in the sample, and also to identify different populations of organisms present in the sample.
Incubate the plates away from light at a temperature of 35±2 for 24-18 hours. If the results are negative after 24 hours, continue the incubation for another 24 hours.

Results and Interpretation

After incubation, the expected results from the growth of organisms are as follows:

Salmonella species, excluding Salmonella typhi and Salmonella paratyphi: white to pink colonies surrounded by a red halo.
Salmonella typhi and Salmonella paratyphi: weak growth or no growth.
Shigella: weak growth or no growth.
E. coli, Enterobacter, and Klebsiella: yellow or greenish-yellow colonies surrounded by a greenish-yellow halo.
Proteus: weak growth or no growth.
Pseudomonas: Pink to reddish colonies.
Gram-positive bacteria: weak growth or no growth.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Dehydrated Appearance:

Dehydrated Appearance: Pink, free-flowing, homogeneous.
Prepared Appearance: Orange-brown, very slightly to slightly opalescent.
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours

Colony color	Growtht	ATCC	Standard Strain
Yellow-green	Weak to good	25922	Escherichia coli
Red	Good	13076	Salmonella enterica subsp. Enterica serotype Enteritidis
Red	None to poor	19430	Salmonella enterica subsp. enterica None to serotype Typhi
Red	Good	14028	Salmonella enterica subsp. Enterica serotype Typhimurium
–	Inhibition	25923	Staphylococcus aureus

Component

gr/Lit	Components
10.0t	Proteose Peptone
3.0	Yeast Extract
10.0t	Lactose
10.0t	Sucrose
5.0	Sodium Chloride
0.0125t	Brilliant Green
0.08t	Phenol Red
12.0	Agar
0.2 ± 6.9t	Final pH

Brilliant Green Agar (BGA)

STORAGE:

Test

Test

Results and Interpretation

Dehydrated Appearance:

STORAGE:

Test

Test

Results and Interpretation

Dehydrated Appearance:

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