LMX Broth, modified

Application: LMX broth is a enrichment medium used for simultaneous identification of coliforms and E. coli in water, milk, and food products.

Catalogue Number: i23217

For more information, please click: Chromogenic and Fluorogenic Culture media

توضیحات

Preparation

Weigh 17 grams of the powder and dissolve it in 1 liter of distilled water.
Heat it gently until the medium boild and powder is completely dissolved.
After the medium is dissolved, pour it into test tubes.
Autoclave at 121°C for 15 minutes.

STORAGE

Store the powdered medium in a tightly sealed container at a temperature below 30 degrees Celsius, and keep the prepared medium at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

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Components

gr/L	Component
5.0	Tryptose
5.0	Sodium Chloride
1.0	Sorbitol
1.0	Tryptophan
2.7	Dipotassium Hydrogen Phosphate
2.0	Potassium Dihydrogen Phosphate
0.1	Lauryl Sulfate Sodium Salt
0.08	X-Gal
0.05	MUG
0.1	IPTG
0.2 ± 6.8	Final pH

Quality Control

Dehydrated Appearance: Cream to light yellow, homogeneous, free-flowing
Prepared Appearance: Cream to light yellow.
Cultural response was observed after 18-48 hours of incubation at 35 ± 0.5˚C.

Indole reaction	Fluorescence	Color change of the medium to green-blue	ATCC	Standard Strain
Positive	Positive	Positive	25922	Escherichia coli
-	Negative	Positive	8090	Citrobacter freundii
-	Negative	Negative	25923	Staphylococcus aureus

Test

Test process

Inoculate the water or food sample onto the culture medium. The testing process depends heavily on the type of sample and the method used in each laboratory.
Incubate the inoculated samples at 35 ± 2°C for 24 hours.

Result and Interpretation

The change in the color of the medium from yellow to blue-green indicates the presence of coliforms. The blue fluorescence under UV light with a wavelength of 366 nm indicates the presence of E. coli.
By adding Kovac’s reagent to the surface of the medium, a red ring is created indicating the presence of E. coli (indole test).

Note

If no fluorescence is observed after 24 hours, do not add Kovac’s reagent and continue incubating for another 24 hours. The fluorescence and indole production should then be examined.

Related products

Cat. Number	Vial Content	Product
iR95008	50 ml / 100 ml	Kovac’s Reagent

توضیحات

Preparation

Weigh 17 grams of the powder and dissolve it in 1 liter of distilled water.
Heat it gently until the medium boild and powder is completely dissolved.
After the medium is dissolved, pour it into test tubes.
Autoclave at 121°C for 15 minutes.

STORAGE

Store the powdered medium in a tightly sealed container at a temperature below 30 degrees Celsius, and keep the prepared medium at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/L	Component
5.0	Tryptose
5.0	Sodium Chloride
1.0	Sorbitol
1.0	Tryptophan
2.7	Dipotassium Hydrogen Phosphate
2.0	Potassium Dihydrogen Phosphate
0.1	Lauryl Sulfate Sodium Salt
0.08	X-Gal
0.05	MUG
0.1	IPTG
0.2 ± 6.8	Final pH

Quality Control

Dehydrated Appearance: Cream to light yellow, homogeneous, free-flowing
Prepared Appearance: Cream to light yellow.
Cultural response was observed after 18-48 hours of incubation at 35 ± 0.5˚C.

Indole reaction	Fluorescence	Color change of the medium to green-blue	ATCC	Standard Strain
Positive	Positive	Positive	25922	Escherichia coli
-	Negative	Positive	8090	Citrobacter freundii
-	Negative	Negative	25923	Staphylococcus aureus

Test

Test process

Inoculate the water or food sample onto the culture medium. The testing process depends heavily on the type of sample and the method used in each laboratory.
Incubate the inoculated samples at 35 ± 2°C for 24 hours.

Result and Interpretation

The change in the color of the medium from yellow to blue-green indicates the presence of coliforms. The blue fluorescence under UV light with a wavelength of 366 nm indicates the presence of E. coli.
By adding Kovac’s reagent to the surface of the medium, a red ring is created indicating the presence of E. coli (indole test).

Note

If no fluorescence is observed after 24 hours, do not add Kovac’s reagent and continue incubating for another 24 hours. The fluorescence and indole production should then be examined.

Related products

Cat. Number	Vial Content	Product
iR95008	50 ml / 100 ml	Kovac’s Reagent

LMX Broth, modified

Preparation

STORAGE

Quality Control

Test process

Result and Interpretation

Note

Related products

Preparation

STORAGE

Quality Control

Test process

Result and Interpretation

Note

Related products

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

Downloading CoA & MSDS files

CoA & MSDS files

Downloading CoA & MSDS files